β-endorphin inhibition of progesterone secretion by porcine granulosa cells during follicle development
نویسندگان
چکیده
منابع مشابه
Porcine granulosa cells produce a progesterone secretion inhibitory activity.
OBJECTIVE To extend our previous observations of granulosa cell conditioned medium inhibition on basal and FSH-stimulated progesterone secretion by granulosa cells from large porcine antral follicles. METHODS Granulosa cell conditioned media (SGCCM) were obtained from cultures of granulosa cells which were harvested from small porcine antral follicles. SGCCM were fractionated by filtration th...
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BACKGROUND Ion channels occur as large families of related genes with cell-specific expression patterns. Granulosa cells have been shown to express voltage-gated potassium channels from more than one family. The purpose of this study was to determine the effects of 4-aminopyridine (4-AP), an antagonist of KCNA but not KCNQ channels. METHODS Granulosa cells were isolated from pig follicles and...
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The effect of porcine follicular fluid on estradiol and progesterone secretion was examined using a rat granulosa cell culture with FSH and testosterone in the medium. Follicular fluids from small (less than 5 mm) and large (greater than 6 mm) follicles (SFFI, LFF1) were treated with charcoal, and then fractionated by filtration through an Amicon XM-50 and an PM-10 membrane. The addition of 25%...
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ژورنال
عنوان ژورنال: Reproduction Nutrition Development
سال: 1998
ISSN: 0926-5287
DOI: 10.1051/rnd:19980302